June 22~26, 2011. UCLA
I was there to give a poster presentation entitled ‘RACK-1 acts in let-7-mediated heterochronic development of Caenorhabditis elegans’ and chair a session for developmental biology.
Well, I looked like a student. I was trying to sell the RACK-1 story.
Session Title: Gene Regulation and Genomics: RNA interference and small RNAs Session Type: Poster
Session Location: Wooden Center – Collins Court Session Time: Thursday Abstract Information
Poster Board Number: 978A Presentation Time: June 23, 2011, THU 8:00PM-9:30PM Abstract Content
RACK-1 acts in let-7-mediated heterochronic development of Caenorhabditis elegans. Shih-Peng Chan1, Frank Slack2. 1) Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei 10051, Taiwan; 2) Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA.
In Caenorhabditis elegans, the let-7 miRNA regulates the exit of cell cycle and terminal differentiation at the L4-to-adult switch. Dysfunction of let-7 results in irregular hypodermal and vulval development of C. elegans and is also a feature of human lung cancer. Here we show that Receptor of Activated C Kinase (RACK-1) modulates let-7-mediated regulation in C. elegans. The RACK-1 protein co-immunoprecipitated with the nematode Argonaute protein, ALG-1. Reduction of rack-1 gene expression by RNAi suppressed the aberrant vulva and hypodermis development phenotypes of let-7(n2853) mutant animals and promoted adult-specific gene expression. Our results indicate a relationship between RACK-1 and let-7 in regulation of terminal differentiation that may help understand the mechanism of translational control by miRNAs.