Lately we are able to generate single-copy insertion dual-color 3’UTR reporter that is expressed in C. elegans seam cells! How exciting!
GFP containing MDOC PEST is controlled by a 3’UTR of interest and mCherry by the unc-54 3’UTR as internal contriol. These two fluorescence genes are driven by the famous SCM promoter and splitted by a gpd-2/3 intergenic region that contains an SL2 transplice site.
Everything is preliminary but we are happy to see that it dose work.